Engine Prime doesn't analyse on OSX Sierra anymore

I have a situation with Engine Prime, after importing Serato tracks, (I used the guidelines from the post of “Lostnthesound”) everything looks fine. After closing Engine prime and restarting 5 minutes later, my tracks are loading slow, analyzing doesn’t work anymore, also there’s no Track or Artist showing. Engine doesn’t store changes anymore. a fresh install of engine prime (with deleting all the Engine prime databases), doesn’t help. My tracks are on a separate fat32 partition. I’m stuck here…

Is there anything running in the background, like a backup, or Time Machine, or other housekeeping?

Only Time Machine with standard settings